Bioelectrochemical systems (BES) offer significant potential for treating refractory waste and recovering bioenergy. However, their ability to mitigate microplastic pollution in wastewater remains unexplored. This study showed that BES facilitated the treatment of polyethylene (PE), polyvinyl chloride (PVC), and Mix (PE+PVC) microplastic wastewater and the methane recovery (40.61%, 20.02%, 21.19%, respectively), associated with higher soluble chemical oxygen demand (SCOD) removal and lower volatile fatty acids (VFAs) accumulation. The lactate dehydrogenase (LDH), adenosine triphosphate (ATP), cytochrome c, and nicotinamide adenine dinucleotide (NADH/NAD+) ratios were elevated with electrical stimulation, and PE decreased reactive oxygen species (ROS) formation, while PVC enhanced it. Moreover, the applied voltage improved the polysaccharides content of the extracellular polymeric substances (EPS) in the PE-BES but decreased in PVC-BES, while the proteins showed the opposite trend. Analysis of the microbial community using 16S sequencing revealed that BES enhanced the diversity and richness of the microbial community. Metatranscriptomic sequencing further showed that the abundance of fermentation bacteria, acetogens, electrogens, and methanogens was greatly enhanced by applying voltage, especially at the anode. Methane metabolism was dominated by the acetoclastic methanogenic pathway, with the applied voltage promoting the enrichment of Methanothrix, resulting in the direct conversion of acetate to acetyl-CoA via acetate-CoA ligase (EC: 6.2.1.1), and increased metabolic activity in the anode. Moreover, applied voltage greatly boosted the function genes expression level related to energy metabolism, tricarboxylic acid (TCA) cycle, electron transport, and transporters on the anode biofilm. Overall, these results demonstrate that BES can mitigate microplastic pollution during wastewater treatment.
 

Bioelectrochemical systems; Mitigate; Microplastic pollution; Wastewater treatment; Methane metabolism